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Also, the test duration is only 90 minutes, with only 4 sample collections required, compared with 2 hours for the ITT and 4 hours for the GST

After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

The interplay between hyperglycemia and oxidative stress contributes to the pathogenesis of diabetic complications, including neuropathy, nephropathy, retinopathy, and cardiovascular diseases [172]

doi: 10.1007/s00508-009-1232-5

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